Supplement to current insights in mycoplasmology. Proceedings of the International Organization for Mycoplasmology 1980-82. Abstracts.

one group of abstracts was inadvertently omitted. In order to complete the formal record of this meeting, we are publishing the following abstracts. The editors hope that, in spite of its lateness, this supplementary publication will prove useful. Canada We can report that both A. hippikon and A. morum S2, like all Acholeplasma species, have NADH oxidase localized in membrane fractions and can also synthesize lipid from acetate. Acholeplasmas were grown in a modified Edwards medium with 1 l2 to 2 percent (vol/vol) heat-reinactivated horse serum [J Bacteriol 146:1055, 1982]. Eight serotypes of Ureaplasma urealyticum grown in B broth [J Clin Microbiol 7:127, 1978] were also examined and none could synthesize lipid from radioactive acetate, a finding in contradiction to an earlier published report. The type strain of U. urealyticum was tested and had no detectable NADH oxidase activity in either membrane or cytoplasmic fractions. Except for this Ureaplasma sp. all other tested Mollicutes are reported to have NADH oxidase activity localized in one or the other fraction. A non-sterol-requiring Mollicutes isolated from lemon by R.E. McCoy had a higher specific activity of NADH oxidase in soluble fractions than in membrane fractions, which is contrary to findings with all other Acholeplasma species. This unexplained reversal of NADH oxidase localization may be related to the serum of the growth medium. The same isolate incorporated less acetate into lipid than any species of Acholeplasma and more than any species of Myco-plasma. Another non-sterol-requiring Mollicutes (Lett 1) isolated from lettuce by N.L. Somer-son had apparently no NADH oxidase activity in either membrane or soluble fractions, and, like the lemon isolate, synthesized less lipid from acetate than any Acholeplasma species but more than any Mycoplasma species. A. hippikon, A. morum, and the non-sterol-requiring lemon and lettuce isolates all had lactic dehydrogenase activity localized in their cytoplasmic fractions. These findings suggest the emergence of a new metabolically distinguishable group of Molli-cutes and the need to examine more isolates, particularly from plants. A. laidlawii strain JAIR differs from the parent strain by being resistant to infection with MVL2; it was selected from A. laidlawii strain JAI as described previously [Al-Shammari AJN, Smith PF: J Virol 36:120, 1980]. SDS polyacrylamide gel electrophoresis of membrane proteins of both strains revealed some differences in protein bands. Soft laser scanning densitometer shows lowered amount of one major protein in the JAIR membrane. Lipid classes of both strains were similar. Attempts …